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Product Info Summary
| SKU: | A00172-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Dnmt1 Antibody Picoband™
SKU/Catalog Number
A00172-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dnmt1 Antibody Picoband™ catalog # A00172-3. Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Dnmt1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00172-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Dnmt1 recombinant protein (Position: Q509-Q1577).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00172-3 is reactive to DNMT1 in Mouse
Applications
A00172-3 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
200 kDa
Calculated molecular weight
183.165kDa
Background of DNMT1
DNA (cytosine-5)-methyltransferase 1 is an enzyme that in humans is encoded by the DNMT1 gene. This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Dnmt1 using anti-Dnmt1 antibody (A00172-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt1 antigen affinity purified polyclonal antibody (Catalog # A00172-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dnmt1 at approximately 200 kDa. The expected band size for Dnmt1 is at 183 kDa.
Protein Target Info & Infographic
Gene/Protein Information For DNMT1 (Source: Uniprot.org, NCBI)
Gene Name
DNMT1
Full Name
DNA (cytosine-5)-methyltransferase 1
Weight
183.165kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
AIM; CXXC9; CXXC9CXXC finger protein 9; CXXC-type zinc finger protein 9; DNA (cytosine-5-)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase 1; DNA methyltransferase HsaI; DNA MTase HsaI; DNMT; DNMT1; EC 2.1.1.37; FLJ16293; m.HsaI; MCMT; MCMTMGC104992 DNMT1 ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI DNA methyltransferase 1 DNA (cytosine-5)-methyltransferase 1|CXXC-type zinc finger protein 9|DNA (cytosine-5-)-methyltransferase 1|DNA MTase HsaI|DNA methyltransferase HsaI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNMT1, check out the DNMT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNMT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dnmt1 Antibody Picoband™ (A00172-3)
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No publications found for A00172-3
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